沙葱多糖对绵羊外周血淋巴细胞IFN-γ与STAT1 mRNA表达的影响

doi:10.11843/j.issn.0366-6964.2013.12.011

Abstract

Abstract:

The experiment was conducted to study the effects of Allium mongolicum Regel polysaccharides on the mRNA expression of IFN-γ and STAT1 in peripheral blood lymphocyte in vitro,and to discuss the possible molecular mechanism of its anti-tumor function in sheep. Different concentrations (0, 50, 100, 150, 200, 250 μg·mL^-1 ) of Allium mongolicum Regel polysaccharides were added in sheep peripheral blood lymphocytes cultures, and total RNA in cells was extracted with β-actin as an internal reference real-time PCR was adopt to test. The changes of relative mRNA expressions of IFN-γ and STAT1 in different culrured times (12, 24, 48, 72 h) were tested by real-time PCR and the PCR products of IFN-γ and STAT1 were sequenced and analyzed. The relative mRNA expressions of IFN-γ and STAT1 in Allium mongolicum Regel polysaccharides treatment were increased significantly (P<0.05) compared with control group (0 μg·mL^-1), and showed an upward trend with the increase of Allium mongolicum Regel polysaccharides concentration. The sequencing analysis proved that the PCR products were IFN-γ and STAT1 gene sequence in sheep. The results suggested that Allium mongolicum Regel polysaccharides could significantly promote the mRNA expression of IFN-γ and STAT1 in sheep peripheral blood lymphocytes of sheep, may thereby play an important role in anti-tumor effect.

CLC Number:

S826
S813.3

ZHAO Fei-yan, AO Chang-jin, BAO Mei-yan, SA Ru-li. Effect of Allium mongolicum Regel Polysaccharides on IFN-γ and STAT1 mRNA Expression in Peripheral Blood Lymphocyte of Sheep[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(12): 1932-1938.

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Effect of Allium mongolicum Regel Polysaccharides on IFN-γ and STAT1 mRNA Expression in Peripheral Blood Lymphocyte of Sheep

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